Plasmid-Mediated Gene Disruption in Streptomyces griseus.
نویسندگان
چکیده
منابع مشابه
Genetic recombination in Streptomyces griseus.
Low-frequency (10(-6)) genetic recombination was observed in a cephamycin-producing strain of Streptomyces griseus. The recombinants were predominantly heteroclones. Heteroclone analysis was performed involving four heteroclones of one cross. In 100 mutants correlation was found between the type of auxotrophy and the level of antibiotic activity. A cross of this strain with a streptomycin-produ...
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The complete nucleotide sequence of the 1525 bp 16S ribosomal RNA gene from the rrnE operon of Streptomyces griseus subsp. griseus KCTC9080 was determined (1). The 5' and 3' ends of the 16S rRNA were located with the combination of previous data (2,3,4) and have not been determined experimentally. The nucleotide sequence presented here was determined following the dideoxy method (5) and using M...
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A wild-type strain of Streptomyces griseus forms spores both on solid media (aerial spores) and in liquid culture (submerged spores). Both spore types are highly resistant to sonication, but only aerial spores are resistant to lysozyme digestion. Electron micrographs suggest that lysozyme sensitivity may result from the thinner walls of the submerged spores. Studies of the life cycle indicate t...
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UV irradiation of Streptomyces griseus 2247 yielded a new chromosomal deletion mutant, MM9. Restriction and sequencing analysis revealed that homologous recombination between two similar lipoprotein-like open reading frames, which are located 450 and 250 kb from the left and right ends, respectively, caused chromosomal arm replacement. As a result, new 450-kb terminal inverted repeats (TIRs) we...
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ژورنال
عنوان ژورنال: Actinomycetologica
سال: 1994
ISSN: 1881-6371,0914-5818
DOI: 10.3209/saj.8_17